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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

NLRX1 Polyclonal antibody

NLRX1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17215-1-AP

Synonyms

Nucleotide-binding oligomerization domain protein 9, Nucleotide-binding oligomerization domain protein 5, NOD9, NOD5, NLRX 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NLRX1 Polyclonal antibody (17215-1-AP)

    WB analysis using 17215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NLRX1 Polyclonal antibody (17215-1-AP)

    WB analysis using 17215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NLRX1 Polyclonal antibody (17215-1-AP)

    WB analysis of HepG2 using 17215-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NLRX1 Polyclonal antibody (17215-1-AP)

    WB analysis of MCF-7 using 17215-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    WB analysis of mouse skeletal muscle using 17215-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IP experiment of mouse heart using 17215-1-AP

    IP result of anti-NLRX1 (IP:17215-1-AP, 3ug; Detection:17215-1-AP 1:800) with mouse heart tissue lysate 3520ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human heart using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human heart using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of mouse heart using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of mouse heart using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human skeletal muscle using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human skeletal muscle using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human liver using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using NLRX1 Polyclonal antibody (17215-1-AP)

    IHC staining of human liver using 17215-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NLRX1 Polyclonal antibody (17215-1-AP)

    IF Staining of HepG2 using 17215-1-AP

    Immunofluorescent analysis of HepG2 cells, using NLRX1 antibody 17215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using NLRX1 Polyclonal antibody (17215-1-AP)

    FC experiment of HepG2 using 17215-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NLRX1 (17215-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue, mouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17215-1-AP targets NLRX1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRX1 fusion protein Ag11000 相同性解析による交差性が予測される生物種
Full Name NLR family member X1
Calculated molecular weight 975 aa, 108 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC013199
Gene symbol NLRX1
Gene ID (NCBI) 79671
RRIDAB_2236031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NLRX1 antibody 17215-1-APDownload protocol
IHC protocol for NLRX1 antibody 17215-1-APDownload protocol
IF protocol for NLRX1 antibody 17215-1-APDownload protocol
IP protocol for NLRX1 antibody 17215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Mitochondrial DNA stress primes the antiviral innate immune response.

Authors - A Phillip West
View Article
humanWB

Mol Cell

Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.

Authors - Samuel A Killackey
  • KD Validated
View Article
humanWB,IHC

J Exp Med

NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.

Authors - Geurt Stokman
  • KO Validated
View Article
human,mouseWB

PLoS Pathog

PMID: 28542569

Authors - Jae-Hoon Kim
View Article
humanWB

Front Immunol

PMID: 30344524

Authors - Tünde Fekete
View Article
mouseIF

Front Immunol

NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.

Authors - Juin-Hua Huang
View Article