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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated NLRX1 Polyclonal antibody (CL594-17215)

    IF Staining of HepG2 using CL594-17215

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 NLRX1 antibody (CL594-17215) at dilution of 1:800.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated NLRX1 Polyclonal antibody (CL594-17215)

    IF Staining of A549 using CL594-17215

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 NLRX1 antibody (CL594-17215) at dilution of 1:800.

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  • KD/KO Validated

CoraLite®594-conjugated NLRX1 Polyclonal antibody

NLRX1 Polyclonal Antibody for IF/ICC
Cat No. CL594-17215

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Nucleotide-binding oligomerization domain protein 9, Nucleotide-binding oligomerization domain protein 5, NOD9, NOD5, NLRX 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17215 targets NLRX1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRX1 fusion protein Ag11000 相同性解析による交差性が予測される生物種
Full Name NLR family member X1
Calculated molecular weight 975 aa, 108 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC013199
Gene Symbol NLRX1
Gene ID (NCBI) 79671
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UT6
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLRX1 (Nucleotide-binding oligomerization domain, leucine-rich repeat containing X1) is a member of the NOD-like receptor (NLR) family and is unique among NLRs due to its localization to the mitochondrial matrix. NLRX1 is a negative regulator of innate immunity, particularly in viral infections. It interacts with the mitochondrial antiviral signaling protein (MAVS) to attenuate antiviral responses. NLRX1 has been implicated in various diseases, including multiple sclerosis, colorectal cancer, and ischemia-reperfusion injury. Its role in controlling inflammation and mitochondrial function makes it a potential therapeutic target.

Protocols

Product Specific Protocols
IF protocol for CL594 NLRX1 antibody CL594-17215Download protocol
Standard Protocols
Click here to view our Standard Protocols