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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NMDAR1/GRIN1 Monoclo (CL488-67717)

    IF Staining of mouse brain using CL488-67717

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NMDAR1/GRIN1 antibody (CL488-67717, Clone: 1H2C2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated NMDAR1/GRIN1 Monoclo (CL488-67717)

    IF Staining of mouse brain using CL488-67717

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 NMDAR1/GRIN1 antibody (CL488-67717, Clone: 1H2C2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CoraLite® Plus 488-conjugated NMDAR1/GRIN1 Monoclonal antibody

NMDAR1/GRIN1 Monoclonal Antibody for IF-P
Cat No. CL488-67717
Clone No.1H2C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken

Applications

IF-P

Synonyms

GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67717 targets NMDAR1/GRIN1 in IF-P applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NMDAR1/GRIN1 fusion protein Ag26364 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Calculated molecular weight 105 kDa
Observed molecular weight105-120 kDa
GenBank accession numberNM_000832
Gene Symbol NMDAR1
Gene ID (NCBI) 2902
RRIDAB_3672960
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NMDAR1/GRIN1 antibody CL488-67717Download protocol
Standard Protocols
Click here to view our Standard Protocols