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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOC2L Polyclonal antibody (28509-1-AP)

    WB analysis using 28509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NOC2L Polyclonal antibody (28509-1-AP)

    WB analysis using 28509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOC2L Polyclonal antibody (28509-1-AP)

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOC2L Polyclonal antibody (28509-1-AP)

    IHC staining of human prostate cancer using 28509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOC2L Polyclonal antibody (28509-1-AP)

    IF Staining of U2OS using 28509-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 28509-1-AP (NOC2L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin (red).

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NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28509-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp564C186, FLJ35172, NIR, NOC2 like protein, NOC2L, Protein NOC2 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, HEK-293T cells, HuH-7 cells, mouse cerebellum tissue, mouse stomach tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28509-1-AP targets NOC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOC2L fusion protein Ag26952 相同性解析による交差性が予測される生物種
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
RRIDAB_2881160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

Protocols

Product Specific Protocols
WB protocol for NOC2L antibody 28509-1-APDownload protocol
IHC protocol for NOC2L antibody 28509-1-APDownload protocol
IF protocol for NOC2L antibody 28509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols