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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated NOC2L Polyclonal ant (CL488-28509)

    IF Staining of U2OS using CL488-28509

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NOC2L antibody (CL488-28509) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated NOC2L Polyclonal ant (CL488-28509)

    FC experiment of U2OS using CL488-28509

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody (CL488-28509)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28509

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Protein NOC2 homolog, Nucleolar complex protein 2 homolog, Novel INHAT repressor, NOC2-like protein, NIR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28509 targets NOC2L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOC2L fusion protein Ag26952 相同性解析による交差性が予測される生物種
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
RRIDAB_3672828
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NOC2L antibody CL488-28509Download protocol
FC protocol for CL Plus 488 NOC2L antibody CL488-28509Download protocol
Standard Protocols
Click here to view our Standard Protocols