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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOM1 Polyclonal antibody (29798-1-AP)

    WB analysis using 29798-1-AP (same clone as 29798-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29798-1-AP (NOM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NOM1 Polyclonal antibody (29798-1-AP)

    IHC staining of mouse cerebellum using 29798-1-AP (same clone as 29798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29798-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NOM1 Polyclonal antibody (29798-1-AP)

    IHC staining of mouse cerebellum using 29798-1-AP (same clone as 29798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29798-1-PBS in a different storage buffer formulation.

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NOM1 Polyclonal antibody, PBS Only

NOM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29798-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29798-1-PBS targets NOM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOM1 fusion protein Ag31423 相同性解析による交差性が予測される生物種
Full Name nucleolar protein with MIF4G domain 1
Calculated molecular weight96KD
Observed molecular weight96~100 kDa
GenBank accession numberNM_138400
Gene Symbol NOM1
Gene ID (NCBI) 64434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5C9Z4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleolar protein NOM1, which contains an MIF4G domain and an MA3 domain, was first isolated from the bone marrow of children with acute myeloid leukemia. NOM1 is highly conserved in a variety of species, including in yeast and in humans.