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CoraLite® Plus 488-conjugated NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55114

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3


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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55114 targets NOTCH3 in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Notch homolog 3 (Drosophila)
Calculated molecular weight 244 kDa
Observed molecular weight 250-260 kDa, 100 kDa
GenBank accession numberNM_000435
Gene symbol NOTCH3
Gene ID (NCBI) 4854
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NOTCH3 antibody CL488-55114Download protocol
Standard Protocols
Click here to view our Standard Protocols