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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NQO2 Polyclonal anti (CL488-15767)

    IF Staining of HeLa using CL488-15767

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NQO2 antibody (CL488-15767) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated NQO2 Polyclonal antibody

NQO2 Polyclonal Antibody for IF/ICC
Cat No. CL488-15767

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

NMOR2, EC:1.10.5.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15767 targets NQO2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NQO2 fusion protein Ag8403 相同性解析による交差性が予測される生物種
Full Name NAD(P)H dehydrogenase, quinone 2
Calculated molecular weight 231 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC006096
Gene Symbol NQO2
Gene ID (NCBI) 4835
RRIDAB_3083976
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16083
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NQO2 antibody CL488-15767Download protocol
Standard Protocols
Click here to view our Standard Protocols