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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR1H4 Recombinant antibody (81820-2-RR)

    WB analysis using 81820-2-RR (same clone as 81820-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81820-2-RR (NR1H4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81820-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NR1H4 Recombinant antibody (81820-2-RR)

    WB analysis of HepG2 using 81820-2-RR (same clone as 81820-2-PBS)

    WB result of NR1H4 antibody (81820-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR1H4 transfected HepG2 cells. This data was developed using the same antibody clone with 81820-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NR1H4 Recombinant antibody (81820-2-RR)

    FC experiment of HepG2 using 81820-2-RR (same clone as 81820-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NR1H4 Recombinant antibody (81820-2-RR, Clone:4B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81820-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81820-2-RR

    Affinity and Kinetic Characterization of 81820-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81820-2-RR against Human NR1H4 were performed. The affinity constant is 0.807 nM.

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  • Featured Product
  • KD/KO Validated

NR1H4 Recombinant antibody, PBS Only

NR1H4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81820-2-PBS
Clone No.4B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Farnesol receptor HRR-1, Farnesoid X-activated receptor, Farnesoid X activated receptor, Bile acid receptor, BAR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81820-2-PBS targets NR1H4 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NR1H4 fusion protein Ag21948 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 1, group H, member 4
Calculated molecular weight 486 aa, 56 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC130573
Gene Symbol NR1H4
Gene ID (NCBI) 9971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RI1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)