- Featured Product
- KD/KO Validated
NR1H4 Recombinant antibody
NR1H4 Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
4B4
Cat no : 81820-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MKN-45 cells, HepG2 cells, HEK-293 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81820-2-RR targets NR1H4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | NR1H4 fusion protein Ag21948 相同性解析による交差性が予測される生物種 |
Full Name | nuclear receptor subfamily 1, group H, member 4 |
Calculated molecular weight | 486 aa, 56 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC130573 |
Gene symbol | NR1H4 |
Gene ID (NCBI) | 9971 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have four isoforms, i.e., FXRalpha1, FXRalpha2, FXRbeta1, and FXRbeta2, and each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NR1H4 antibody 81820-2-RR | Download protocol |
FC protocol for NR1H4 antibody 81820-2-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |