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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NR2C2 Monoclonal antibody (67489-1-Ig)

    WB analysis of HeLa using 67489-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67489-1-Ig (NR2C2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NR2C2 Monoclonal antibody (67489-1-Ig)

    WB analysis of HEK-293 using 67489-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67489-1-Ig (NR2C2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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NR2C2 Monoclonal antibody

NR2C2 Monoclonal Antibody for WB, ELISA
Cat No. 67489-1-Ig
Clone No.2E4C10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

hTAK1, NR2C2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, TAK1, Testicular receptor 4, TR2R1, TR4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67489-1-Ig targets NR2C2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group C, member 2
Calculated molecular weight 65 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberNM_003298
Gene Symbol NR2C2
Gene ID (NCBI) 7182
RRIDAB_2882715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NR2C2, also named as TAK1, TR4 and TR2R1, belongs to the nuclear hormone receptor family and NR2 subfamily. NR2C2 is an orphan nuclear receptor. It may regulate gene expression during the late phase of spermatogenesis.

Protocols

Product Specific Protocols
WB protocol for NR2C2 antibody 67489-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols