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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRBP2 Monoclonal antibody

NRBP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4F10H7

Cat no : 66687-1-Ig

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRBP2 Monoclonal antibody (66687-1-Ig)

    WB analysis using 66687-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NRBP2 Monoclonal antibody (66687-1-Ig)

    WB analysis using 66687-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRBP2 Monoclonal antibody (66687-1-Ig)

    IHC staining of mouse brain using 66687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRBP2 Monoclonal antibody (66687-1-Ig)

    IHC staining of mouse brain using 66687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NRBP2 Monoclonal antibody (66687-1-Ig)

    IF Staining of HEK-293 using 66687-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, A549 cells, HeLa cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66687-1-Ig targets NRBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NRBP2 fusion protein Ag17011 相同性解析による交差性が予測される生物種
Full Name nuclear receptor binding protein 2
Calculated molecular weight 501 aa, 58 kDa
Observed molecular weight 58 kDa, 33 kDa
GenBank accession numberBC113873
Gene symbol NRBP2
Gene ID (NCBI) 340371
RRIDAB_2882041
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for NRBP2 antibody 66687-1-IgDownload protocol
IHC protocol for NRBP2 antibody 66687-1-IgDownload protocol
IF protocol for NRBP2 antibody 66687-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols