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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRBP2 Monoclonal antibody, PBS Only

NRBP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F10H7

Cat no : 66687-1-PBS

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NRBP2 Monoclonal antibody (66687-1-Ig)

    WB analysis using 66687-1-Ig (same clone as 66687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NRBP2 Monoclonal antibody (66687-1-Ig)

    WB analysis using 66687-1-Ig (same clone as 66687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRBP2 Monoclonal antibody (66687-1-Ig)

    IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRBP2 Monoclonal antibody (66687-1-Ig)

    IHC staining of mouse brain using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NRBP2 Monoclonal antibody (66687-1-Ig)

    IF Staining of HEK-293 using 66687-1-Ig (same clone as 66687-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66687-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66687-1-PBS targets NRBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NRBP2 fusion protein Ag17011 相同性解析による交差性が予測される生物種
Full Name nuclear receptor binding protein 2
Calculated molecular weight 501 aa, 58 kDa
Observed molecular weight 58 kDa, 33 kDa
GenBank accession numberBC113873
Gene symbol NRBP2
Gene ID (NCBI) 340371
RRIDAB_2882041
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.