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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRCAM Monoclonal antibody

NRCAM Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2E1C3

Cat no : 68291-1-Ig

Synonyms

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis using 68291-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis of rat cerebellum using 68291-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis of pig brain using 68291-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    IF Staining of mouse brain using 68291-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, pig cerebellum tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68291-1-Ig targets NRCAM in WB, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRCAM fusion protein Ag20558 相同性解析による交差性が予測される生物種
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC098401
Gene symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

Protocols

Product Specific Protocols
WB protocol for NRCAM antibody 68291-1-IgDownload protocol
IF protocol for NRCAM antibody 68291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols