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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis using 68291-1-Ig (same clone as 68291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis of rat cerebellum using 68291-1-Ig (same clone as 68291-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    WB analysis of pig brain using 68291-1-Ig (same clone as 68291-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRCAM Monoclonal antibody (68291-1-Ig)

    IF Staining of mouse brain using 68291-1-Ig (same clone as 68291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68291-1-PBS in a different storage buffer formulation.

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NRCAM Monoclonal antibody, PBS Only

NRCAM Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 68291-1-PBS
Clone No.2E1C3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF-P, Indirect ELISA

Synonyms

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68291-1-PBS targets NRCAM in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRCAM fusion protein Ag20558 相同性解析による交差性が予測される生物種
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC098401
Gene Symbol NRCAM
Gene ID (NCBI) 4897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92823
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).