NRF1/nuclear respiratory factor 1 Monoclonal antibody
NRF1/nuclear respiratory factor 1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
3C2B8
Cat no : 66832-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
Product Information
66832-1-Ig targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NRF1/nuclear respiratory factor 1 fusion protein Ag28149 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear respiratory factor 1 |
| Calculated molecular weight | 522 aa, 56 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC016925 |
| Gene symbol | NRF1 |
| Gene ID (NCBI) | 4899 |
| RRID | AB_2882175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-Ig | Download protocol |
| IHC protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-Ig | Download protocol |
| IF protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Transl Hepatol Icaritin Attenuates Lipid Accumulation by Increasing Energy Expenditure and Autophagy Regulated by Phosphorylating AMPK. | ||
PLoS One Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats. | ||
Nitric Oxide Hydrogen sulfide alleviates heart failure with preserved ejection fraction in mice by targeting mitochondrial abnormalities via PGC-1α | ||
J Pharm Pharmacol Styrax (Liquidambar orientalis Mill.) promotes mitochondrial function and reduces cardiac damage following myocardial ischemic injury: the role of the AMPK-PGC1α signaling pathway | ||
Folia Biol (Praha) NRF1 Alleviated Oxidative Stress of Glioblastoma Cells by Regulating NOR1 | ||
Anim Biotechnol Efficacy of creatine nitrate supplementation on redox status and mitochondrial function in pectoralis major muscle of preslaughter transported broilers |
