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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRF1/nuclear respiratory factor 1 Monoclonal antib (66832-1-Ig)

    WB analysis using 66832-1-Ig (same clone as 66832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66832-1-Ig (NRF1/nuclear respiratory factor 1) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NRF1/nuclear respiratory factor 1 Monoclonal antib (66832-1-Ig)

    IHC staining of human colon cancer using 66832-1-Ig (same clone as 66832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66832-1-Ig (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NRF1/nuclear respiratory factor 1 Monoclonal antib (66832-1-Ig)

    IF Staining of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (66832-1-Ig, Clone: 3C2B8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NRF1/nuclear respiratory factor 1 Monoclonal antib (66832-1-Ig)

    FC experiment of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human NRF1/nuclear respiratory factor 1 (66832-1-Ig, Clone:3C2B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

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NRF1/nuclear respiratory factor 1 Monoclonal antibody, PBS Only

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66832-1-PBS
Clone No.3C2B8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66832-1-PBS targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149 相同性解析による交差性が予測される生物種
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2882175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.