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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NRF1/nuclear respiratory factor 1 Recombinant antibody

NRF1/nuclear respiratory factor 1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230394B1

Cat no : CL488-83092

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated NRF1/nuclear respira (CL488-83092)

    IF Staining of HepG2 using CL488-83092

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NRF1/nuclear respiratory factor 1 antibody (CL488-83092, Clone: 230394B1 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83092 targets NRF1/nuclear respiratory factor 1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172 相同性解析による交差性が予測される生物種
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016925
Gene symbol NRF1
Gene ID (NCBI) 4899
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NRF1/nuclear respiratory factor 1 antibody CL488-83092Download protocol
Standard Protocols
Click here to view our Standard Protocols