Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, HepG2 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, rabbit brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
66492-1-Ig targets NRG1, isoform SMDF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat, rabbit samples.
| Tested Reactivity | Human, pig, mouse, rat, rabbit |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NRG1, isoform SMDF fusion protein Ag0803 相同性解析による交差性が予測される生物種 |
| Full Name | neuregulin 1 |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC007675 |
| Gene Symbol | NRG1 |
| Gene ID (NCBI) | 3084 |
| RRID | AB_2881857 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02297 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRG1, isoform SMDF antibody 66492-1-Ig | Download protocol |
| IF protocol for NRG1, isoform SMDF antibody 66492-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Prog Neuropsychopharmacol Biol Psychiatry The Notch1/Hes1 pathway regulates Neuregulin 1/ErbB4 and participates in microglial activation in rats with VPA-induced autism | ||
Int J Mol Sci Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory |