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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    WB analysis using 66492-1-Ig (same clone as 66492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    WB analysis of pig cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    WB analysis of rat cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    WB analysis of HepG2 using 66492-1-Ig (same clone as 66492-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    WB analysis of mouse cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NRG1, isoform SMDF Monoclonal antibody (66492-1-Ig)

    IF Staining of MCF-7 using 66492-1-Ig (same clone as 66492-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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NRG1, isoform SMDF Monoclonal antibody, PBS Only

NRG1, isoform SMDF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66492-1-PBS
Clone No.2C6D12

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66492-1-PBS targets NRG1, isoform SMDF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, mouse, rat, rabbit samples.

Tested Reactivity Human, pig, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NRG1, isoform SMDF fusion protein Ag0803 相同性解析による交差性が予測される生物種
Full Name neuregulin 1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
RRIDAB_2881857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02297
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.