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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRG1, isoform Alpha Polyclonal antibody (31043-1-AP)

    WB analysis using 31043-1-AP (same clone as 31043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NRG1, isoform Alpha Polyclonal antibody (31043-1-AP)

    IF Staining of MCF-7 using 31043-1-AP (same clone as 31043-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 31043-1-PBS in a different storage buffer formulation.

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NRG1, isoform Alpha Polyclonal antibody, PBS Only

NRG1, isoform Alpha Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31043-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31043-1-PBS targets NRG1, isoform Alpha in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRG1, isoform Alpha fusion protein Ag35114 相同性解析による交差性が予測される生物種
Full Name neuregulin 1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02297
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.