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CoraLite® Plus 488-conjugated NSD3 Polyclonal antibody
NSD3 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11345
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11345 targets NSD3 in FC (Intra) applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSD3 fusion protein Ag1894 相同性解析による交差性が予測される生物種 |
| Full Name | Wolf-Hirschhorn syndrome candidate 1-like 1 |
| Calculated molecular weight | 1437 aa, 162 kDa |
| Observed molecular weight | 160-170 kDa, 68-72 kDa |
| GenBank accession number | BC012059 |
| Gene symbol | WHSC1L1 |
| Gene ID (NCBI) | 54904 |
| RRID | AB_2919027 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 NSD3 antibody CL488-11345 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
