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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 647-conjugated NSFL1C Monoclonal an (CL647-68182)

    IF Staining of A431 using CL647-68182

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 NSFL1C antibody (CL647-68182, Clone: 1F12E12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated NSFL1C Monoclonal an (CL647-68182)

    FC experiment of A431 using CL647-68182

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody (CL647-68182, Clone:1F12E12)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-68182
Clone No.1F12E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-68182 targets NSFL1C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NSFL1C fusion protein Ag8040 相同性解析による交差性が予測される生物種
Full Name NSFL1 (p97) cofactor (p47)
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC002801
Gene Symbol NSFL1C
Gene ID (NCBI) 55968
RRIDAB_3673647
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNZ2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
FC protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
Standard Protocols
Click here to view our Standard Protocols