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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NUDT21 Monoclonal antibody

NUDT21 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C4C8

Cat no : CL488-66335

Synonyms

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, DKFZp686H1588, Nudix motif 21, NUDT21

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated NUDT21 Monoclonal an (CL488-66335)

    IF Staining of HepG2 using CL488-66335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66335 (NUDT21 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66335 targets NUDT21 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag12260 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001403
Gene symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2883312
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NUDT21 antibody CL488-66335Download protocol
Standard Protocols
Click here to view our Standard Protocols