Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK293 cells, HeLa cells, ROS1728 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
66359-1-Ig targets NUP155 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NUP155 fusion protein Ag3638 相同性解析による交差性が予測される生物種 |
| Full Name | nucleoporin 155kDa |
| Calculated molecular weight | 1391 aa, 155 kDa |
| Observed molecular weight | 140-155 kDa |
| GenBank accession number | BC039257 |
| Gene Symbol | NUP155 |
| Gene ID (NCBI) | 9631 |
| RRID | AB_2881738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| IHC protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
J Ethnopharmacol Xinfuli granule alleviates metabolic remodeling through inhibition of endoplasmic reticulum stress and mitochondrial injury in heart failure | ||
BMC Cancer Pan-cancer analysis of NUP155 and validation of its role in breast cancer cell proliferation, migration, and apoptosis
| ||
Front Pharmacol NUP155 and NDC1 interaction in NSCLC: a promising target for tumor progression |