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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of HEK293 cells using 66359-1-Ig (same clone as 66359-1-PBS)

    WB result of NUP155 antibody (66359-1-Ig, 1: 6000) with si-Control and si-NUP155 transfected HEK293 cells. This data was developed using the same antibody clone with 66359-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of HeLa using 66359-1-Ig (same clone as 66359-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66359-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of ROS1728 using 66359-1-Ig (same clone as 66359-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66359-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP155 Monoclonal antibody (66359-1-Ig)

    IHC staining of human breast cancer using 66359-1-Ig (same clone as 66359-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66359-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP155 Monoclonal antibody (66359-1-Ig)

    IHC staining of human breast cancer using 66359-1-Ig (same clone as 66359-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66359-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NUP155 Monoclonal antibody, PBS Only

NUP155 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66359-1-PBS
Clone No.1A7F2

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66359-1-PBS targets NUP155 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NUP155 fusion protein Ag3638 相同性解析による交差性が予測される生物種
Full Name nucleoporin 155kDa
Calculated molecular weight 1391 aa, 155 kDa
Observed molecular weight 140-155 kDa
GenBank accession numberBC039257
Gene Symbol NUP155
Gene ID (NCBI) 9631
RRIDAB_2881738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75694
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.