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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP62 Monoclonal antibody

NUP62 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12B3

Cat no : 66573-1-Ig

Synonyms

Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN, 62 kDa nucleoporin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis using 66573-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis of RAW 264.7 using 66573-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, Raji cells, DU 145 cells, PC-3 cells, HepG2 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66573-1-Ig targets NUP62 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUP62 fusion protein Ag4912 相同性解析による交差性が予測される生物種
Full Name nucleoporin 62kDa
Calculated molecular weight 522 aa, 53 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC014842
Gene symbol NUP62
Gene ID (NCBI) 23636
RRIDAB_2881933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Protocols

Product Specific Protocols
WB protocol for NUP62 antibody 66573-1-IgDownload protocol
IHC protocol for NUP62 antibody 66573-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols