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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis using 66573-1-Ig (same clone as 66573-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using NUP62 Monoclonal antibody (66573-1-Ig)

    WB analysis of RAW 264.7 using 66573-1-Ig (same clone as 66573-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NUP62 Monoclonal antibody (66573-1-Ig)

    IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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NUP62 Monoclonal antibody, PBS Only

NUP62 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66573-1-PBS
Clone No.2G12B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN, 62 kDa nucleoporin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66573-1-PBS targets NUP62 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUP62 fusion protein Ag4912 相同性解析による交差性が予測される生物種
Full Name nucleoporin 62kDa
Calculated molecular weight 522 aa, 53 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC014842
Gene Symbol NUP62
Gene ID (NCBI) 23636
RRIDAB_2881933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP37198
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)