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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated NUSAP1 Polyclonal an (CL488-12024)

    IF Staining of HeLa using CL488-12024

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NUSAP1 antibody (CL488-12024) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated NUSAP1 Polyclonal antibody

NUSAP1 Polyclonal Antibody for IF/ICC
Cat No. CL488-12024

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ANKT, BM037, FLJ13421, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12024 targets NUSAP1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUSAP1 fusion protein Ag2654 相同性解析による交差性が予測される生物種
Full Name nucleolar and spindle associated protein 1
Calculated molecular weight 440 aa, 49 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC001308
Gene Symbol NUSAP1
Gene ID (NCBI) 51203
RRIDAB_3083911
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXS6
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NUSAP1 antibody CL488-12024Download protocol
Standard Protocols
Click here to view our Standard Protocols