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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using NXF2 Polyclonal antibody (12185-1-AP)

    WB analysis of mouse testis using 12185-1-AP (same clone as 12185-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12185-1-AP (NXF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12185-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using NXF2 Polyclonal antibody (12185-1-AP)

    IP experiment of mouse testis using 12185-1-AP (same clone as 12185-1-PBS)

    IP result of anti-NXF2 (IP:12185-1-AP, 4ug; Detection:12185-1-AP 1:400) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NXF2 Polyclonal antibody (12185-1-AP)

    IHC staining of human lymphoma using 12185-1-AP (same clone as 12185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12185-1-AP (NXF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12185-1-PBS in a different storage buffer formulation.

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NXF2 Polyclonal antibody, PBS Only

NXF2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12185-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12185-1-PBS targets NXF2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NXF2 fusion protein Ag2841 相同性解析による交差性が予測される生物種
Full Name nuclear RNA export factor 2
Calculated molecular weight 626 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC015020
Gene Symbol NXF2
Gene ID (NCBI) 56001
RRIDAB_2236531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZY0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NXF2, also named as Nuclear RNA export factor 2 or TAPL2, is a 626 amino acid protein, which contains one TAP-C domain, one RRM domain, one NTF2 domain, and four LRR repeats. NXF2 belongs to the NXF family and is expressed almost exclusively in testis.NXF2 localizes in the nucleus and is involved in the export of mRNA from the nucleus to the cytoplasm.