Validation Data Gallery
Product Information
Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB |
| Host | Alpaca-derived, recombinantly produced in bacteria |
| Species Reactivity | Mouse IgG1 Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3, IgM |
| Target/Specificity | Fc-fragment of mouse IgG1 |
| Physical State | Liquid |
| Conjugate | CoraLite® Plus 488 |
| Suggested Dilution | Immunofluorescence 1:500 Western blot 1: 500 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG2a, IgG2b, IgG2c, IgG3, and IgM |
| Immunogen | Purified mouse IgG1 |
| Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
| Clones | CTK0103 (VHH0302), CTK104 (VHH0305) |
| RRID | AB_2941310 |
| Affinity (KD) | CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM |
| Excitation / Emission | Excitation max: 490 nm, Emission max: 525 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C. |
| Size | 10 μL; 100 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| smsG1CL488-1_SDS_Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 488 [CTK0103, CTK0104] (EN) |
| Datasheet |
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| Nano-Secondary® anti-mouse IgG1, recombinant VHH, CoraLite® Plus 488 [CTK0103, CTK0104] Datasheet |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
| Application note |
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| One-step Incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
Publications
| Application | Title |
|---|---|
ACS Omega Early Exposure of an Infantile Rat to Sex-Related Content Induces Precocious Puberty by Activation of Cholinergic Neurons in the Amygdala and KNDy Neurons in the Arcuate Nucleus | |
Mol Neurobiol Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay |