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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    IF Staining of MCF-7 using 83223-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    FC experiment of MCF-7 using 83223-4-RR

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 83223-4-RR

    Affinity and Kinetic Characterization of 83223-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

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Nectin-4/PVRL4 Recombinant antibody

Nectin-4/PVRL4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC, ELISA
Cat No. 83223-4-RR
Clone No.240009F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC, ELISA

Synonyms

Nectin-4, PVRL4, Nectin cell adhesion molecule 4, nectin 4, LNIR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83223-4-RR targets Nectin-4/PVRL4 in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=2.28 x 10-10M
KOff=2.98 x 10-5M
KOn=2.62 x 105M
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 4
Calculated molecular weight 510 aa, 55 kDa
GenBank accession numberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
RRIDAB_3670907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Protocols

Product Specific Protocols
IF protocol for Nectin-4/PVRL4 antibody 83223-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols