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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    IF Staining of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    FC experiment of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1, Nectin-4/PVRL4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-4-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

  • Affinity and Kinetic Characterization of 83223-4-RR

    Affinity and Kinetic Characterization of 83223-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

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Nectin-4/PVRL4 Recombinant antibody, PBS Only (Detector)

Nectin-4/PVRL4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC, Cytometric bead array, Indirect ELISA
Cat No. 83223-4-PBS
Clone No.240009F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC, Cytometric bead array, Indirect ELISA

Synonyms

Nectin-4, PVRL4, Nectin cell adhesion molecule 4, nectin 4, LNIR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83223-4-PBS targets Nectin-4/PVRL4 as part of a matched antibody pair:

MP00147-1: 83223-1-PBS capture and 83223-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=2.28 x 10-10M
KOff=2.98 x 10-5M
KOn=2.62 x 105M
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 4
Calculated molecular weight 510 aa, 55 kDa
GenBank accession numberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).