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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nesprin 3 Monoclonal antibody, PBS Only

Nesprin 3 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1G12

Cat no : 68420-1-PBS

Synonyms

C14orf49, FLJ25605, Nesprin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nesprin 3 Monoclonal antibody (68420-1-Ig)

    WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Nesprin 3 Monoclonal antibody (68420-1-Ig)

    IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68420-1-PBS targets Nesprin 3 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nesprin 3 fusion protein Ag26012 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 49
Observed molecular weight110 kDa
GenBank accession numberBC146604
Gene symbol C14orf49
Gene ID (NCBI) 161176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).