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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G12

Cat no : 68420-1-Ig

Synonyms

C14orf49, FLJ25605, Nesprin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nesprin 3 Monoclonal antibody (68420-1-Ig)

    WB analysis using 68420-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Nesprin 3 Monoclonal antibody (68420-1-Ig)

    IF Staining of mouse testis using 68420-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, C2C12 cells, PC-12 cells, rat testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68420-1-Ig targets Nesprin 3 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nesprin 3 fusion protein Ag26012 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 49
Observed molecular weight110 kDa
GenBank accession numberBC146604
Gene symbol Nesprin 3
Gene ID (NCBI) 161176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Protocols

Product Specific Protocols
WB protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
IF protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols