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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D1G12

Cat no : CL488-68420

Synonyms

C14orf49, FLJ25605, Nesprin 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 488-conjugated Nesprin 3 Monoclonal (CL488-68420)

    IF Staining of mouse testis using CL488-68420

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 Nesprin 3 antibody (CL488-68420, Clone: 1D1G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68420 targets Nesprin 3 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nesprin 3 fusion protein Ag26012 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 49
Observed molecular weight110 kDa
GenBank accession numberBC146604
Gene symbol C14orf49
Gene ID (NCBI) 161176
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Nesprin 3 antibody CL488-68420Download protocol
Standard Protocols
Click here to view our Standard Protocols