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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated Neurotrophin 4 Polyc (CL488-12297)

    IF Staining of PC-3 using CL488-12297

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 Neurotrophin 4 antibody (CL488-12297) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Neurotrophin 4 Polyclonal antibody

Neurotrophin 4 Polyclonal Antibody for IF/ICC
Cat No. CL488-12297

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

neurotrophin 4, Neurotrophin 5, Neutrophic factor 4, NT 4, NT 4/5, NT 5, NT4, NT-4, NT5, NTF4, NTF5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12297 targets Neurotrophin 4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurotrophin 4 fusion protein Ag2938 相同性解析による交差性が予測される生物種
Full Name neurotrophin 4
Calculated molecular weight 210 aa, 22 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC012421
Gene Symbol Neurotrophin 4
Gene ID (NCBI) 4909
RRIDAB_3672534
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP34130
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Neurotrophin 4 antibody CL488-12297Download protocol
Standard Protocols
Click here to view our Standard Protocols