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Validation Data Gallery

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  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 647-conjugated Noggin Monoclonal an (CL647-69010)

    FC experiment of PC-3 using CL647-69010

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Noggin (CL647-69010, Clone:2A11C1) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647-conjugated Noggin Monoclonal antibody

Noggin Monoclonal Antibody for FC (Intra)
Cat No. CL647-69010
Clone No.2A11C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

NOG, SYNS1, SYM1, 2A11C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-69010 targets Noggin in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Noggin fusion protein HZ-1118 相同性解析による交差性が予測される生物種
Full Name noggin
Gene Symbol Noggin
Gene ID (NCBI) 9241
RRIDAB_2920322
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles. This antibody is used to Block/Neutralize Noggin protein.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Noggin antibody CL647-69010Download protocol
Standard Protocols
Click here to view our Standard Protocols