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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NeutraKine®Thrombopoietin Monoclonal antibody

NeutraKine®Thrombopoietin Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

2H8G1

Cat no : 69028-1-Ig

Synonyms

C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO

Validation Data Gallery

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  • Neutralization experiment of NeutraKine®Thrombopoietin using NeutraKine®Thrombopoietin Monoclonal antibody (69028-1-Ig)

    Neutralization experiment of NeutraKine®Thrombopoietin using 69028-1-Ig

    Recombinant human Thrombopoietin (Cat.NO. HZ-1248) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Thrombopoietin (30 ng/mL HZ-1248) is neutralized by mouse anti-human Thrombopoietin monoclonal antibody 69028-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.5μg/mL.The NeutraControl mouse anti-human Thrombopoietin antibody 69528-1-Ig could recognize human Thrombopoietin but could not neutralize human Thrombopoietin acitivity in the range of 0.00122-20 μg/mL (green curve, refer to top X-right Y axis).

  • Neutralization experiment of NeutraKine®Thrombopoietin using NeutraKine®Thrombopoietin Monoclonal antibody (69028-1-Ig)

    Neutralization experiment of NeutraKine®Thrombopoietin using 69028-1-Ig

    Recombinant human Thrombopoietin (Cat.NO. HZ-1248) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Thrombopoietin (30 ng/mL HZ-1248) is neutralized by mouse anti-human Thrombopoietin monoclonal antibody 69028-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.5μg/mL.

  • ELISA experiment of Recombinant protein using NeutraKine®Thrombopoietin Monoclonal antibody (69028-1-Ig)

    ELISA experiment of Recombinant protein using 69028-1-Ig

    Indirect ELISA was carried out by coating recombinant Human Thrombopoietin (Cat.NO. HZ-1248) at 70 ng/well followed by blocking and adding serial diluted Thrombopoietin antibody 69028-1-Ig and 69528-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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NeutralizationTF-1 cell

Product Information

69028-1-Ig targets NeutraKine®Thrombopoietin in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human HumankineThrombopoietin protein HZ-1248 相同性解析による交差性が予測される生物種
Full Name thrombopoietin
Gene symbol Thrombopoietin
Gene ID (NCBI) 7066
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

This antibody can be used to neutralize the bioactivity of Thrombopoietin.