Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13409-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13409-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13409-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 13409-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13409-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 13409-1-AP
IP result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug.
IHC staining of human breast cancer using 13409-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13409-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13409-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13409-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 13409-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 13409-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13409-1-AP

Publications(474)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

OCLN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (474)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, mouse liver tissue, human kidney tissue, human liver tissue, mouse colon tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 379 publications below
IHC	See 67 publications below
IF	See 31 publications below
IP	See 2 publications below

Product Information

13409-1-AP targets Occludin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Occludin fusion protein Ag4057 相同性解析による交差性が予測される生物種
Full Name	occludin
Calculated molecular weight	522 aa, 59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC029886
Gene Symbol	Occludin
Gene ID (NCBI)	4950
RRID	AB_2156308
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16625
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 13409-1-AP	Download protocol
IHC protocol for Occludin antibody 13409-1-AP	Download protocol
IF protocol for Occludin antibody 13409-1-AP	Download protocol
IP protocol for Occludin antibody 13409-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway Authors - Yanan Zhang View Article
mouse	IHC	Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation Authors - Zhenyu Tian View Article
human	WB	Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression Authors - Xingran Li View Article
human	WB	Hepatology Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress. Authors - Meng Lu View Article
mouse	WB	J Exp Med Brain endothelial TAK1 and NEMO safeguard the neurovascular unit. Authors - Dirk A Ridder View Article
human	WB	Nat Commun Daxx inhibits hypoxia-induced lung cancer cell metastasis by suppressing the HIF-1α/HDAC1/Slug axis. Authors - Ching-Wen Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13409-1-AP

WB analysis of mouse liver using 13409-1-AP

WB analysis of mouse liver using 13409-1-AP

WB analysis of mouse liver using 13409-1-AP

WB analysis of human kidney using 13409-1-AP

WB analysis of human liver using 13409-1-AP

IP experiment of mouse liver using 13409-1-AP

IHC staining of human breast cancer using 13409-1-AP

IHC staining of human breast cancer using 13409-1-AP

IHC staining of human breast cancer using 13409-1-AP

IHC staining of human breast cancer using 13409-1-AP

IF Staining of MCF-7 using 13409-1-AP

IF Staining of MCF-7 using 13409-1-AP

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.

Daxx inhibits hypoxia-induced lung cancer cell metastasis by suppressing the HIF-1α/HDAC1/Slug axis.