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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Occludin Polyclonal antibody

Occludin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13409

Synonyms

occludin, OCLN

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using CoraLite®594-conjugated Occludin Polyclonal antibo (CL594-13409)

    IF Staining of T-47D using CL594-13409

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated Occludin Polyclonal antibo (CL594-13409)

    IF Staining of MCF-7 using CL594-13409

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:600.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells, T-47D cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-13409 targets Occludin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057 相同性解析による交差性が予測される生物種
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
GenBank accession numberBC029886
Gene symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2919795
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
IF protocol for CL594 Occludin antibody CL594-13409Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mucosal Immunol

Stress systems exacerbate the inflammatory response after corneal abrasion in sleep-deprived mice via the IL-17 signaling pathway

Authors - Yunxia Xue
View Article