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- KD/KO Validated
ODF2 Polyclonal antibody
ODF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12058-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, mouse lung tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| IHC | See 1 publications below |
| IF | See 25 publications below |
Product Information
12058-1-AP targets ODF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ODF2 fusion protein Ag2693 相同性解析による交差性が予測される生物種 |
| Full Name | outer dense fiber of sperm tails 2 |
| Calculated molecular weight | 763 aa, 89 kDa |
| Observed molecular weight | 67-100 kDa |
| GenBank accession number | BC010629 |
| Gene symbol | ODF2 |
| Gene ID (NCBI) | 4957 |
| RRID | AB_2156630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ODF2 antibody 12058-1-AP | Download protocol |
| IHC protocol for ODF2 antibody 12058-1-AP | Download protocol |
| IF protocol for ODF2 antibody 12058-1-AP | Download protocol |
| IP protocol for ODF2 antibody 12058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Cell Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility. | ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
J Exp Med A DNAH17 missense variant causes flagella destabilization and asthenozoospermia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jay (Verified Customer) (12-02-2019) | Methanol Fixation.Immunostaining result shows that Odf2 specifically marks the mother centriole in ciliated NIH3T3 cell (Odf2;red, GT335;green for primary cilia, DAPI;blue for nucleus).
 |
