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CoraLite® Plus 488-conjugated ODF2 Polyclonal antibody
ODF2 Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-12058
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-12058 targets ODF2 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ODF2 fusion protein Ag2693 相同性解析による交差性が予測される生物種 |
| Full Name | outer dense fiber of sperm tails 2 |
| Calculated molecular weight | 763 aa, 89 kDa |
| Observed molecular weight | 67-100 kDa |
| GenBank accession number | BC010629 |
| Gene symbol | ODF2 |
| Gene ID (NCBI) | 4957 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 ODF2 antibody CL488-12058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
