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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OGDH Monoclonal antibody, PBS Only

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7A2

Cat no : 66285-1-PBS

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of ROS1728 using 66285-1-Ig (same clone as 66285-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OGDH Monoclonal antibody (66285-1-Ig)

    IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66285-1-PBS targets OGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen OGDH fusion protein Ag7484 相同性解析による交差性が予測される生物種
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC004964
Gene symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.