Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, ROS1728 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
66285-1-Ig targets OGDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | OGDH fusion protein Ag7484 相同性解析による交差性が予測される生物種 |
| Full Name | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| Calculated molecular weight | 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC004964 |
| Gene Symbol | OGDH |
| Gene ID (NCBI) | 4967 |
| RRID | AB_2881668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02218 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OGDH antibody 66285-1-Ig | Download protocol |
| IHC protocol for OGDH antibody 66285-1-Ig | Download protocol |
| IF protocol for OGDH antibody 66285-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. | ||
PLoS One Targeting pancreatic cancer with combinatorial treatment of CPI-613 and inhibitors of lactate metabolism. | ||
Nat Cardiovasc Res α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction |