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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated OGDH Monoclonal antibody

OGDH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7A2

Cat no : CL488-66285

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated OGDH Monoclonal anti (CL488-66285)

    IF Staining of HepG2 using CL488-66285

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:200, CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CoraLite® Plus 488-conjugated OGDH Monoclonal anti (CL488-66285)

    IF Staining of L02 using CL488-66285

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:100, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66285 targets OGDH in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen OGDH fusion protein Ag7484 相同性解析による交差性が予測される生物種
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC004964
Gene symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_2883303
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 OGDH antibody CL488-66285Download protocol
Standard Protocols
Click here to view our Standard Protocols