Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, human cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
Product Information
66513-1-Ig targets OLIG2 (for WB only) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | OLIG2 (for WB only) fusion protein Ag18838 相同性解析による交差性が予測される生物種 |
| Full Name | oligodendrocyte lineage transcription factor 2 |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 35-39 kDa |
| GenBank accession number | BC047511 |
| Gene Symbol | OLIG2 |
| Gene ID (NCBI) | 10215 |
| RRID | AB_2881876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13516 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OLIG2 (for WB only) antibody 66513-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK | ||
Cell Biochem Funct Ring finger protein 216 loss-of-function induces white matter hyperintensities by inhibiting oligodendroglia proliferation | ||
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Commun Biol Selenoprotein W modulates tau homeostasis in an Alzheimer's disease mouse model |