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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OLIG2 Monoclonal antibody, PBS Only

OLIG2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E8C5

Cat no : 66513-1-PBS

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using OLIG2 (for WB only) Monoclonal antibody (66513-1-Ig)

    WB analysis of human brain using 66513-1-Ig (same clone as 66513-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66513-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human cerebellum tissue using OLIG2 (for WB only) Monoclonal antibody (66513-1-Ig)

    WB analysis of human cerebellum using 66513-1-Ig (same clone as 66513-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66513-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66513-1-PBS targets OLIG2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OLIG2 fusion protein Ag18838 相同性解析による交差性が予測される生物種
Full Name oligodendrocyte lineage transcription factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 35-39 kDa
GenBank accession numberBC047511
Gene symbol OLIG2
Gene ID (NCBI) 10215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )