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OPA1 Monoclonal antibody

OPA1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66583-1-Ig
Clone No.1B2D8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, pig, rat and More (2)

Applications

WB, IHC, IF, ELISA

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, HeLa cells, HepG2 cells, Y79 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66583-1-Ig targets OPA1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, fish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen OPA1 fusion protein Ag26868 相同性解析による交差性が予測される生物種
Full Name optic atrophy 1 (autosomal dominant)
Calculated molecular weight 960 aa, 112 kDa
Observed molecular weight 100 kDa and 80-90 kDa
GenBank accession numberBC075805
Gene Symbol OPA1
Gene ID (NCBI) 4976
RRIDAB_2881943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Protocols

Product Specific Protocols
WB protocol for OPA1 antibody 66583-1-IgDownload protocol
IHC protocol for OPA1 antibody 66583-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Genes Dis

TNFα-reliant FSP1 up-regulation promotes intervertebral disc degeneration via caspase 3-dependent apoptosis

Authors - Cheng Qiu
mouseWB,IF

J Agric Food Chem

Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner

Authors - Yanli Zhang
mouseWB

J Endocrinol

ChREBP-β regulates thermogenesis in brown adipose tissue.

Authors - Chunchun Wei
fishWB

J Physiol Sci

Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart

Authors - Zhanglin Chen
WB

Behav Brain Res

Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice.

Authors - Donghyun Kim
human,mouseWB

Biochim Biophys Acta Mol Basis Dis

SENP1 prevents high fat diet-induced non-alcoholic fatty liver diseases by regulating mitochondrial dynamics

Authors - Wenjing Zeng
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