Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-60293 targets OPTN/Optineurin in WB, IHC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | OPTN/Optineurin fusion protein Ag1272 相同性解析による交差性が予測される生物種 |
| Full Name | optineurin |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 70-74 kDa |
| GenBank accession number | BC013876 |
| Gene Symbol | OPTN |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2883065 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96CV9 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin OPTN/Optineurin antibody Biotin-60293 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |